isolation and identification of helicobacter pullorum from caecal content of broiler chickens in mashhad, iran

نویسندگان

a. jamshidi

m. r. bassami

h. salami

s. mohammadi

چکیده

the presence of helicobacter pullorum in intestinal tract of broiler chickens may be a potential risk for human health. in this study, a total of 100 caecal samples of broilers carcasses from 20 flocks at a poultry abattoir in mashhad suburb were tested for the presence of h. pullorum using modified conventional culture method by combination of culturing on brucella sheep blood agar and a filtering technique. suspected colonies were determined as h. pullorum using polymerase chain reaction (pcr) by amplifying a 447 bp fragment of the 16srrna gene of this bacteria. 41% of caecal content samples and samples from 12 broiler flocks (60%) were determined as positive for the presence of h. pullorum. this is the first report of h. pullorum in iranian poultry flocks. the results showed high prevalence of this bacterium in broiler chickens in this area of iran. it seems using combination of conventional culture method and pcr assay based on amplification from conserved genes allows reliable detection and identification of h. pullorum.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and identification of Helicobacter pullorum from caecal content of broiler chickens in Mashhad, Iran

The presence of Helicobacter pullorum in intestinal tract of broiler chickens may be a potential risk for human health. In this study, a total of 100 caecal samples of broilers carcasses from 20 flocks at a poultry abattoir in Mashhad suburb were tested for the presence of H. pullorum using modified conventional culture method by combination of culturing on Brucella sheep blood agar and a filte...

متن کامل

Helicobacter pullorum in Chickens, Belgium

A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obta...

متن کامل

Isolation, identification, and antimicrobial susceptibility of Clostridium perfringens isolates from acute necrotic enteritis of broiler chickens

The aim of this study was to isolate, identify and determine the antimicrobial susceptibility of Clostridium perfringens (CP) isolates from acute necrotic enteritis of broiler chickens. All broiler carcasses diagnosed as necrotic enteritis (NE) were sampled, subjected to microbial tests and 40 isolates were identified according to standard procedures. The antimicrobial susceptibility of CP isol...

متن کامل

Prevalence of Helicobacter pullorum in broiler chickens reared in intensive and extensive farms

Helicobacter pullorum infections are underestimated due to phenotypic similarities between the genera Helicobacter and Campylobacter. The aim of this work was to estimate the Helicobacter pullorum prevalence in 209 caecum contents collected from Italian broilers reared in 33 intensive and 9 extensive farms by using a Steele-McDermott membrane filter method modified. The isolate identifications ...

متن کامل

synthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides

کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 15

شماره 2 2014

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023